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Je to už docela dlouho, co jsem napsal příspěvek na blog, ale nutno říct, že jsem k tomu měl pádný důvod: nejdřív prostě nebylo co psát, ostatně jako většinu druháku. Potom už začalo být co psát, ale to zase nebyl čas, protože zkouška z fyziologie. A nakonec zkouška z biochemie, takže zase žádný čas, žádná chuť, jenom učení. I když…

Normální příprava na zkoušku u mě opravdu vypadá tak, že se celý den jenom učím, večer si naleju vínko, zahraju si nějakou hru a hurá na kutě, abych příští den v šest ráno zase mohl pokračovat ve studiu. U chemie jsem si ale řekl, že to zkusím netradičně a dám si na přípravu tolik času, že budu moct hrát i přes den a ničemu to vadit nebude. Jak se ale ukázalo, tahle forma studia nebyla ani zdaleka tak efektivní, jak jsem si původně myslel: poměr učení a zábavy se mi totiž velice rychle odchýlil od původního plánu a já najednou zjistil, že si užívám celkem fajn prázdniny, během kterých se holt párkrát denně nutím učit.

Nicméně jsem to tímhle způsobem dokopal až ke zkoušce (času na přípravu jsem měl opravdu hodně) a už se šlo na písemný test. První otázka: co je to hapten. Hm, to jsem někde slyšel, to je něco s imunitou, ne? No jo, to je sice fajn, že ho umím aspoň rámcově zařadit, jenže když se všechny čtyři nabídnuté odpovědi týkají imunity, tak je vám taková znalost na prd.

Druhá otázka: vyberte správné tvrzení o dvojfázovém mechanismu reakce. Hm, co je to dvojfázový mechanismus reakce? Že by mechanismus, kterým probíhá reakce ve dvou fázích? Jaké jsou možnosti: vzniká během něj binární komplex. Vzniká během něj ternární komplex. Do prčic, co to je za slova, vždyť já je v životě neslyšel! Možná jsem tomu učení měl nakonec věnovat o něco víc času; vždyť takhle neprojdu ani předzkouškovým testem!

Nakonec se ale ukázalo, že zbylých 18 otázek bylo docela jednoduchých, dvojfázový mechanismus reakce jsem navíc tipnul správně, takže hapten nehapten, hurá na ústní kolo! Vzorce pyridinu a galaktózy jsem celkem zvládnul, takže nastal čas na první otázku: patologické konformace proteinů. Myslel jsem si, že hlavním gró tohohle tématu bude Alzheimer a amyloid, ale nějak jsme ho přeskákali a rovnou šup na priony a kmen kuru, na kterém byly objeveny. Odvyprávěl jsem celý příběh tak, jak nám ho kdysi podal pan profesor Sedláček (ano, takže genetika!) a šlo se dál: Huntingtonova chorea. Prý jak se dědí, jestli dominantně, nebo recesivně. Jestli autozomálně, nebo gonozomálně. Jaká mutace ji způsobuje. (ano, takže zasegenetika!)

Druhá otázka byla kompartmentace buňky. Měl jsem připravený květnatý seznam všech drah, které v mitochondrii probíhají, stihl jsem ale zmínit jenom dýchací řetězec a Krebsův cyklus a už se mě pan profesor ptal na mitochondriální DNA. Kolik nese genů. Co kódují. Jaké můžou způsobovat potíže jejich mutace. (takže ano, potřetí toho dne zase genetika!)

Pak jsem se s panem profesorem ještě stihl pohádat o tom, že ve spermii opravdu jsou mitochondrie, zatímco on mi tvrdil opak (takže histologie), popsal jsem mu vznik lysozomu (takže zase histologie) a už mě posílal se známkou pryč. Ještě se mě ale jen tak pro jistotu zeptal, co štěpí kyselá lipáza. Tuky. Ano, a kde? V esterové vazbě. Ano, a kolik má esterových vazeb ester cholesterolu? Jednu, protože má jenom jednu hydroxylovou skupinu. No tak paráda, můžete jít.

Sečteno a podtrženo – moje zkouška z biochemie: 2/3 genetiky, 1/3 histologie a teprve, když už jsem měl známku v indexu, přišla jedna otázka z biochemie. Takže děcka: učte se poctivě úplně všechno, protože nikdy nevíte, z čeho vás nakonec vyzkouší!

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